Journal: International Journal of Molecular Imaging

Article Title: In Vitro and Ex Vivo Evaluation of Smart Infra-Red Fluorescent Caspase-3 Probes for Molecular Imaging of Cardiovascular Apoptosis

doi: 10.1155/2011/413290

Figure Lengend Snippet: (a) Typical flow cytometry signal obtained in HME cells. Cells were either untreated (control) or incubated with staurosporine 1 μ M for 6 h, and percentage of cell death, derived from the flow cytometry analysis, obtained in control or staurosporine-treated HME cells. Data are mean ± SEM ( n = 30). (b) Caspase-3 activity in HME cells. Cells were either untreated (control) or treated with staurosporine in the absence or the presence of the caspase-3 inhibitor Z-Asp(OMe)-Gln-Met-Asp(OMe)-CH 2 F (100 μ M). R.L.U.: relative luminescent units. Data are mean ± SEM ( n = 6). ** P < .01 versus controls; † P < .05 versus staurosporine alone.

Article Snippet: HME cells were cultured in 8-well slide chambers (Labtek; Becton Dickinson, Lane Cove, New South Wales, Australia).

Techniques: Flow Cytometry, Control, Incubation, Derivative Assay, Activity Assay

Journal: International Journal of Molecular Imaging

Article Title: In Vitro and Ex Vivo Evaluation of Smart Infra-Red Fluorescent Caspase-3 Probes for Molecular Imaging of Cardiovascular Apoptosis

doi: 10.1155/2011/413290

Figure Lengend Snippet: (a) Activation of QCASP3.2 by recombinant caspases. R.F.U.: relative fluorescent units. Cleavage by caspases 3 and 7 in the absence or presence of the caspase inhibitor Ac-Asp-Glu-Val-Asp-CHO (250 nM) is shown. No fluorescence was detected with caspases 1, 6, 8, and 11, thus these data are not presented. (b) Activation of QCASP3.2 by lysates of HME cells. Cells were either untreated (control) or treated with staurosporine 1 μ M for 6 h, in the absence or the presence of the caspase-3 inhibitor Z-Asp(OMe)-Gln-Met-Asp(OMe)-CH 2 F (100 μ M). R.F.U.: relative fluorescent units. Data are mean ± SEM ( n = 3). ** P < .01 versus control and †† P < .01 versus staurosporine alone by repeated measures ANOVA. (c) Colocalization of activated QCASP3.2-induced fluorescence (yellow) with caspase-3 IF (red) in HME cells. Cells were treated or not with staurosporine 1 μ M for 6 hours, in the absence or the presence of the caspase-3 inhibitor Z-Asp(OMe)-Gln-Met-Asp(OMe)-CH 2 F (100 μ M). Nuclei are stained with Hoechst and appear blue. Orange staining is the colocalization of QCASP3.2 (yellow) with caspase-3 mAb (red; magnification x20).

Article Snippet: HME cells were cultured in 8-well slide chambers (Labtek; Becton Dickinson, Lane Cove, New South Wales, Australia).

Techniques: Activation Assay, Recombinant, Fluorescence, Control, Staining

Journal: International Journal of Molecular Imaging

Article Title: In Vitro and Ex Vivo Evaluation of Smart Infra-Red Fluorescent Caspase-3 Probes for Molecular Imaging of Cardiovascular Apoptosis

doi: 10.1155/2011/413290

Figure Lengend Snippet: Colocalization of activated QASP4.1- and QCASP5.1-induced fluorescence (yellow) with caspase-3 IF (red) in HME cells. Cells were either untreated (control) or treated with staurosporine 1 μ M for 6 h, in the absence or the presence of the caspase-3 inhibitor Z-Asp(OMe)-Gln-Met-Asp(OMe)-CH 2 F (100 μ M). Nuclei are stained with Hoechst and appear blue. The orange staining represents the colocalization of QCASP4.1 or QCASP5.1 (yellow) with caspase-3 mAb (red; magnification x20).

Article Snippet: HME cells were cultured in 8-well slide chambers (Labtek; Becton Dickinson, Lane Cove, New South Wales, Australia).

Techniques: Fluorescence, Control, Staining

Journal: International Journal of Molecular Imaging

Article Title: In Vitro and Ex Vivo Evaluation of Smart Infra-Red Fluorescent Caspase-3 Probes for Molecular Imaging of Cardiovascular Apoptosis

doi: 10.1155/2011/413290

Figure Lengend Snippet: Flow cytometry analysis of Annexin V, QCASP4.1, and QCASP5.1 fluorescence in HME cells. Cells were either untreated (control) or treated with staurosporine 1 μ M for 6 h, in the absence or the presence of the caspase-3 inhibitor Z-Asp(OMe)-Gln-Met-Asp(OMe)-CH 2 F (100 μ M). ** P < .01 versus control; † P < .05, †† P < .01 versus staurosporine alone. Only cells displaying either an FL1 or an FL4 intensity, corresponding respectively to annexin V or QCASP staining, ranging from almost 10² to 10 4 for both SSC and FSC parameters were included in the analysis gate.

Article Snippet: HME cells were cultured in 8-well slide chambers (Labtek; Becton Dickinson, Lane Cove, New South Wales, Australia).

Techniques: Flow Cytometry, Fluorescence, Control, Staining